Department of Pediatrics №2
Scientific work
The person responsible for the scientific work of the Department of Pediatrics No. 2 is Maria Petrovna Prokhorova, Doctor of Medical Sciences, Professor, Professor of the Department of Pediatrics No. 2.
carrying out planned research work of the department
- participation in the work of specialized academic councils
- carrying out dissertation research
- publishing scientific monographs
- publishing scientific articles and abstracts
- presentations at scientific forums
Scientific work of the Department of Pediatrics 2023-2025.
Scientific topic of the department: “Diagnosis and treatment of allergic diseases in children.” Term of completion: 2023-2026.
Allergic diseases are now reaching pandemic proportions worldwide, with prevalence rates of 50% or more in some areas (C. Mastrorilli et al., 2017, O.P. Volosovets et al., 2021, Marushko Y.V. et al., 2022). It has been established that official statistics on the Ukrainian population are not comparable with global indicators of the incidence of allergic pathologies, which may indicate unsatisfactory detection of diseases and inadequate treatment of patients (T.R. Umanets, 2019).
According to WHO forecasts, allergic diseases will soon take first place in the structure of general pathology. Thus, according to statistics, over the past 30 years, the incidence of bronchial asthma, food allergies, and AD has doubled. According to WHO information, one in five people on Earth has an allergic disease [Niankovsky S. L., 2010]. The first manifestation of allergic pathology in children is usually AD [Umanets T.R., 2018].
Research objective: to improve diagnosis and increase the effectiveness of treatment for children with allergic diseases by predicting clinical course and treatment effectiveness.
Scientific value and relevance of the results obtained (scientific and technical products), their comparison with Ukrainian and/or the best foreign analogues:
For the first time, a study was conducted on polymorphism in the CLEC7A and TLR2 genes, expression of the Dectin-1 pattern recognition receptor in the buccal epithelium, and an algorithm was developed for individual pathogenetic therapy of patients with AD, taking into account polymorphism in the CLEC7A and TLR2 genes and the existing sensitization profile. For the first time, the study found that the presence of the rs4696480 polymorphism in the TLR2 gene contributes to a more severe AD phenotype, food sensitization, and carriage or infection with S. aureus. TLR2 probably has an indirect effect on the severity of AD: the recognition and presentation of food molecules and S. aureus antigens by the skin and intestinal mucosa leads to sensitization in allergy-prone children. 1. The presence of the rs4696480 polymorphism in the TLR2 gene contributes to a more severe phenotype of atopic dermatitis (OR=6.395, CI 1.240-32.991), food sensitization (OR=2.88, CI 1.027-8.076) and carriage or infection with S. aureus (OR=7.000, CI 1.852-26.462): recognition and presentation of food molecules and S. aureus antigens by dendritic cells of the skin and intestine leads to sensitization of allergy-prone children.
The significance of polymorphism rs4696480 in the TLR2 gene in the development of allergic diseases has been studied previously, with conflicting results. Data from Salpietro C [Salpietro C, 2011] suggest that the TLR2 -16934 allele may be a genetic factor in susceptibility to AD. The direct molecular mechanism underlying this clinical observation remains unknown. The -16934T allele may increase TLR2 expression on the surface of antigen-presenting cells through a possible regulatory molecular effect of the -16934A>T polymorphism, leading to a reduced risk of asthma or allergic disease [Eder W, 2004]. Indeed, Potaczek et al [Potaczek DP, 2010] found that reporter constructs carrying the -16934T allele exhibit greater transcriptional activity than those carrying the -16934A allele. It is likely that the T allele binds nuclear proteins that correspond to some unknown transcription activating factor [Potaczek DP, 2010]. It can be assumed that other TLR2 polymorphisms associated with the -16934T allele may contribute to the development of AD.
Practical value of the results for the needs of defense, security, economy, and/or society of Ukraine:
In children from the risk group, it is advisable to determine the rs4696480 polymorphism in the TLR2 gene, the presence of S. aureus and Malassezia species colonization on the affected skin areas, and the presence of sensitization to food allergens, Malassezia, and CDP.
In the complex therapy of atopic dermatitis in the presence of a pathological homozygous genotype of the rs4696480 polymorphism in the TLR2 gene, it is recommended to determine food sensitization and the presence of S. aureus colonization of the skin, followed by an individual approach to therapy, taking into account the significant allergen and pathogen.
Department publications in 2023
Published: 15 articles. Of these, 11 were published in Scopus and 4 in professional journals of the Higher Education Accreditation Commission of the Ministry of Education and Science of Ukraine. Abstracts: 1 foreign, 4 at Ukrainian conferences.
Reports: 33, 14 at conferences with international participation, 6 abroad at the European Congress on Allergology and Clinical Immunology (Germany).
Scientific forums organized by the department: XXV All-Ukrainian Scientific and Practical Conference with international participation “Topical Issues in Pediatrics” (Sidelnikov Readings) 20-22.09.2023
Textbook
Volosovets O.P., Kryvopustov S.P., Loginova I.O., Shevtsova T.I. Emergency conditions in neonatology. Emergency medicine. Emergency (rapid) medical care: textbook / I.S. Zozulia, A.O. Volosovets, O.G. Shekera et al. — 5th edition, Medicine Publishing House, Kyiv — 2023. — pp. 409-412.
Defended dissertation for the degree of Doctor of Philosophy
Kryvopustova M.V. “Predicting the effectiveness of treatment and clinical course of bronchial asthma in school-age children sensitized to cat allergens.” 15.09.23 Doctor of Science/PhD. Supervisor: Head of the Department of Pediatrics No. 2, Corresponding Member of the National Academy of Medical Sciences of Ukraine Volosovets O.P.
Defended dissertation for the degree of Doctor of Medical Sciences
Mozyrska O.V. “Atopic dermatitis in children with polymorphisms of pattern recognition receptor genes: clinical course, treatment, and prognosis depending on the sensitization profile.” Specialty 14.01.10 Pediatrics. 15.12.2023. Scientific advisor: Head of the Department of Pediatrics No. 2, Corresponding Member of the National Academy of Medical Sciences of Ukraine Volosovets O.P.
2024
Published: 18 articles. Of these, 16 were published in Scopus and 2 in professional journals of the Higher Education Accreditation Commission of the Ministry of Education and Science of Ukraine. Abstracts: 5 at Ukrainian conferences. Published: 2 manuals.
Reports: 40, 13 at conferences with international participation, 2 abroad at the European Congress on Allergology and Clinical Immunology (Valencia, Spain).
The student club has 14 members. Five reports at student conferences, two abstracts published.
Scientific forums organized by the department: XXVI All-Ukrainian Scientific and Practical Conference “Current Issues in Pediatrics” (Sidelnikov Readings) 19-20.09.2024
Manuals
- Complex patients in pediatrics. Teaching manual. / Edited by G.S. Senatorova/. “Medicine.” Kharkiv. Kharkiv National Medical University. 2024 – 268 p. ISBN978-966-2094-57-2
- Pediatrics. (Neonatology. Hematology, Endocrinology). Teaching and methodological manual / Edited by O.P. Volosovets/. – Kyiv: MEDPRINT, 2023. – 432 p.
2025
Published: 24 articles. Of these, 14 were published in Scopus and 10 in professional journals of the Higher Education Accreditation Commission of the Ministry of Education and Science of Ukraine. Two abstracts were presented at Ukrainian conferences. Two manuals were published.
Reports: 40, 20 at conferences with international participation, 2 abroad at the European Congress on Allergology and Clinical Immunology (Glasgow)
Scientific forums organized by the department:
- All-Ukrainian Scientific and Practical Conference “Pediatrics 2025. New Year’s Meetings” 17.01.2025
- XXVII All-Ukrainian Scientific and Practical Conference “Current Issues in Pediatrics” dedicated to the memory of Corresponding Member of the National Academy of Sciences, Academy of Medical Sciences of Ukraine, Professor V.M. Sidelnikov (Sidelnikov Readings) 17-20.09.2025
Manuals
- Kryvopustov S.P., Volosovets O.P., Kryvopustova M.V., Yuryev S.D. Allergies in children. Clinical and molecular aspects. Textbook. – Kyiv: VSV “Medicine”, 2025. – 151 p. ISBN978-617-8347-02-4
- Nutrition for Children in the First Years of Life. Volosovets O.P., Khomenko V.E., Kryvopustov S.P., Mozirskaya O.V., Prokhorova M.P., Yemets O.V., Karulina Yu.V. Textbook. Kyiv: Medprint, 2025.-100 p. ISBN978-9660370-287-2
Section in a collective monograph
Cymbalyuk V.I., Abdullaev R.Ya., Lazorishinets V.V., Kovalenko V.M., Bilovol O.M., Lurin I.A., Rudenko A.V., Antipkin Yu.G., Volosovets O.P. Visualization of congenital malformations. Kharkiv. 2025. 314 p.
